My queens are reared here in Polk County, Iowa. Most of my bees are of Carniolan origin, but because of open-mating, I don’t claim then to be Carniolan. You can call them “locally-reared mid-western stock” or just good mutts. Queens are reared from strong colonies exhibiting a list of required traits including: Iowa winter-ability, honey production, pest & disease resistance, and gentleness.
We are small-scale with this, maintaining only about 20 – 25 mating nucs this year for queen & nuc production. I try to keep them available from late-spring (May) through late summer (mid-August).
Selling queens is challenging & fun, but right now I don’t really have any intention of “going bigger”. I guess I’m not much of a businessman — I’d love to see more & more beekeepers work to increase the availability of locally-grown queens as part of a sustainable beekeeping model.